Quick KO?»ùÒò±à¼ÊÔ¼ÁºÐÊÇÒ»¿îרΪ¿ÆÑÐÓû§¶¨ÖÆÑз¢µÄall-in-one ¼´ÓÃÐÍCRISPRÇóý²Ù×÷ÊÔ¼ÁºÐ¡£¡£¡£¡£¡£¡£ÆäÄÚ°üÀ¨ÁËCRISPRÇóýËùÐèµÄ£¬£¬£¬£¬£¬£¬£¬£¬´ÓsgRNAÉè¼Æµ½»ñµÃÇóýϸ°ûÖ꣬£¬£¬£¬£¬£¬£¬£¬Íê³ÉʵÑéµÄÖ÷ÒªÖÊÁÏ¡£¡£¡£¡£¡£¡£ÔÚÍê³É»ùÒò±à¼ÊµÑéµÄͬʱ£¬£¬£¬£¬£¬£¬£¬£¬´ó´óÌá¸ß¿ÆÑÐЧÂÊ¡£¡£¡£¡£¡£¡£
¿ÉÔÚ²úÆ·ÊÖÒÕÎļþÖÐÏÂÔØ²úÆ·ÁÐ±í£¬£¬£¬£¬£¬£¬£¬£¬ËÑË÷Ïà¹Ø»ùÒòλµãÐÅÏ¢ÅÌÎÊÁϺ𣡣¡£¡£¡£¡£
²úÆ·ÌØµã
? All-in-one Éè¼Æ£¬£¬£¬£¬£¬£¬£¬£¬ÊµÏÖһվʽʵÑé
? Ò×ÉÏÊÖ£¬£¬£¬£¬£¬£¬£¬£¬±ã½Ý²Ù×÷Íê³ÉʵÑé
? ÎÞÐè¹¹½¨ÖÊÁ££¬£¬£¬£¬£¬£¬£¬£¬½ÚԼʵÑéʱ¼äºÍ¼ò»¯Á÷³Ì
? ÂÄÀúÖ¤µÄsgRNA£¬£¬£¬£¬£¬£¬£¬£¬Ìá¸ßʵÑéÀÖ³ÉÂÊ
? ÎÞÐèÌáÈ¡ºÍ´¿»¯DNA£¬£¬£¬£¬£¬£¬£¬£¬¿ìËÙÑé֤ϸ°û°Ð»ùÒò»ùÒòÐÍ
? ½µµÍʧ°ÜΣº¦£¬£¬£¬£¬£¬£¬£¬£¬½ÚԼʵÑ鱾Ǯ
¼´ÓÃÐÍÊÔ¼Á¡¢¼ò»¯²Ù×÷
Quick-KO?»ùÒòÇóýÊÔ¼ÁºÐ°üÀ¨Òѹ¹½¨ºÃµÄ±í´ïsgRNAºÍCas9µÄÖÊÁ££¨»òÂý²¡¶¾£©¼°»ùÒòÐÍÅжÏÒýÎ£¬£¬£¬£¬£¬£¬£¬¿Í»§¿ÉÒÔÖ±½ÓʹÓ㬣¬£¬£¬£¬£¬£¬£¬¼òÆÓÉÏÊÖ¡£¡£¡£¡£¡£¡£

ÓÅ»¯Éè¼Æ¡¢¸ßЧÇóý
Quick-KO?µÄÇóýЧÂÊÊǹŰ嵥sgRNA»ùÒòÇóýÕ½ÂÔµÄ3~5±¶£¬£¬£¬£¬£¬£¬£¬£¬ÄÜ»ñµÃ¶à¸ö¡°µÈλ»ùÒò¡±ÍêÈ«Çóýϸ°ûÖê¡£¡£¡£¡£¡£¡£Ëü½ÓÄÉÁËÒ»ÖÖÓÅ»¯µÄ¶àµ¼ ÏòÕ½ÂÔ£¬£¬£¬£¬£¬£¬£¬£¬Í¨¹ýsgRNAÖ¸µ¼Cas9¶ÔÌØ¶¨»ùÒòλµã¾ÙÐÐÇи£¬£¬£¬£¬£¬£¬£¬±¬·¢DNAË«Á´¶ÏÁÑ£¨Double-Strand Break£¬£¬£¬£¬£¬£¬£¬£¬DSB£©£¬£¬£¬£¬£¬£¬£¬£¬ÓÕµ¼Æ¬¶Ïȱʧ£¬£¬£¬£¬£¬£¬£¬£¬Ôì ³É»ùÒò¹¦Ð§µÄÇóý¡£¡£¡£¡£¡£¡£Í¬Ê±Quick KO?Ëù°üÀ¨sgRNAÒÑÔÚ293Tϸ°ûÉϾÓɲâÊÔºÍÑéÖ¤£¬£¬£¬£¬£¬£¬£¬£¬Ìá¸ßÁËʵÑéÀÖ³ÉÂÊ¡£¡£¡£¡£¡£¡£
Á½ÖÖ±ê¼Ç¡¢ÎÞаѡÔñ
Quick-KO?»ùÒòÇóýÊÔ¼ÁºÐÄÚº¬sgRNA´øÓÐÓ«¹â£¨mCherry£©ºÍҩɸ£¨puro£©Ë«Öرê¼Ç£¬£¬£¬£¬£¬£¬£¬£¬Óû§¿ÉÒÔÆ¾Ö¤ÐèÒª×ÔÐÐÑ¡Ôñ¡£¡£¡£¡£¡£¡£Ó«¹â±ê¼Ç±ã
ÓÚʹÓÃÁ÷ʽϸ°ûÊõ¶ÔתȾϸ°û¾ÙÐзÖÑ¡£¡£¡£¡£¡£¡£¬£¬£¬£¬£¬£¬£¬£¬¹ØÓÚʹÓÃÁ÷ʽδ±ãµÄÓû§£¬£¬£¬£¬£¬£¬£¬£¬¿ÉʹÓÃҩɸ±ê¼Ç£¬£¬£¬£¬£¬£¬£¬£¬×èÖ¹Á÷ʽ·ÖÑ¡£¡£¡£¡£¡£¡£¬£¬£¬£¬£¬£¬£¬£¬Àû±ãºóÐøÊµÑé¡£¡£¡£¡£¡£¡£
½ÚԼʱ¼ä¡¢Ëõ¶ÌÖÜÆÚ£¬£¬£¬£¬£¬£¬£¬£¬×î¿ì½öÐè4ÖÜ
